mRNA specific amplification by Primer Digestion Amplification (PDA)
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چکیده
منابع مشابه
Genotyping by Allele - Specific Primer Amplification
It has been shown that the Rhesus (Rh) blood group antigens are encoded by two homologous genes: the Rh D gene and the Rh CcEe gene. The Rh CcEe gene encodes different peptides: the Rh C, c, E, and e polypeptides. Only one nucleotide difference has been found between the alleles encoding the Rh E and the Rh e antigen polypeptides. It is a C -+ G transition at nucleotide position 676, which lead...
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Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
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invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. an early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. recently, the polymerase chain reaction (pcr) has been used successfully in detecting specific dna of several pathogen. in this study, nested pcr was used to detect dna specific for a!.pergiflus s...
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The application of DNA as a functional material such as DNA hydrogel has attracted much attention. Despite an increasing interest, the high cost of DNA synthesis is a limiting factor for its utilization. To reduce the cost, we report here a highly efficient amplification technique for polypod-like structured DNA (polypodna) with adhesive ends that spontaneously forms DNA hydrogel. Two types of ...
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“megaprimer method” of site-directed mutagenesis. BioTechniques 8:404-407. 11.Senanayake, S.D. and D.A. Brian. 1995. Precise large deletions by the PCR based overlap extention. Mol. Biotechnol. 4:13-15. 12.Silver, J., T. Limjoco, and Feinstone. 1995. Site-specific mutagenesis using the polymerase chain reaction, p. 179-188. In M.A. Innis, D.H. Gelfand and J.J. Sninsky (Eds.), PCR Strategies. Ac...
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ژورنال
عنوان ژورنال: Japanese Journal of Thrombosis and Hemostasis
سال: 1996
ISSN: 1880-8808,0915-7441
DOI: 10.2491/jjsth.7.330